The Commission had voted to approve sports wagering in January and has now finalised all rules and regulations to do with the vertical.
Resorts World Catskills, Rivers Casino and Resort Schenectady, Tioga Downs Casino Resort and Del Lago Resort and Casino will all offer sports betting as previously planned.
The licensing process will now begin, with the upstate casinos likely to be awarded a license later this summer, before the next NFL season.
Tribal casinos also have plans to offer sports wagering, although mobile wagering within the state is yet to be approved.
Governor Andrew Cuomo has opposed mobile sports betting in New York, while Senator Joseph Addabbo Jr recently revised his proposed mobile bill to include in-stadium wagering.
