Operators SuperSport, Germania and Arena Casino are the first to go live with NetEnt in Croatia, offering the supplier's slot games and marketing tools to players.
This follows NetEnt's entries into the Switzerland and Colombia markets last month.
The provider is to go live with further Croatian operators in the coming months.
NetEnt Malta Managing Director, Henrik Fagerlund, said: "We’ve been eying the Croatian market for a while and we are of course happy to make this market entry.
"We are excited to put our portfolio of slot games in front of players and I’m certain they will enjoy them."
Last month, NetEnt saw its Q1 2020 revenue rise 24% year-on-year to SEK 518m ($52.3m), after fully integrating with Red Tiger during the quarter to help "realise further syngergies."
Red Tiger was acquired in September 2019, in an all-cash deal with an initial value of £197m ($245.3m).
