The card will enable players to remit funds into their gaming accounts and provide access to winnings.
The launch is expected this summer, following necessary regulatory approvals, with more details on the launch expected to follow.
This realtionship could be expanded if Bet365 moves into other US states; the operator is prepared for a New Jersey launch through its partnership with Hard Rock International, announced last July.
The Bet365 card will be the 13th branded, reloadable and prepaid program EML is managing in the gambling industry across Australia, Europe and the US.
Share prices in EML rose to A$1.99 (US$1.41) from A$1.80 through the day, following the announcement.
As the regulated US sports betting market outside Nevada is relatively new, EML is unable to accurately estimate the gross debit volume likely to be made from the programme.